Brush border enzymuria originating from proximal renal tubules as sifted test in patients with psoriatic arthritis

Aim: To compare diagnostic values and laboratory variables of alanine aminopeptidase (microsomale AAP), γ-glutamyl transferase (γGT), β2-microglobuline (β2-M), C Reactive Protein (CRP) and index for disease activity (PASI) in early diagnosis in previously untreated Psoriatic arthritis (Psa). To determine the effect of untreated Psoriatic arthritis on tubular function, sensitivity of the Brush Border region as well as the diagnostic value of the enzymes originating from proximal renal tubules. Methods: From the standard methods of the International Federation for Clinical Chemistry (IFCC) we used the kinetic method for determination of alanine aminopeptidase (microsomal AAP), γ-glutamyl transferase (γGT) and MEIA (Microparticles Enzyme Immunoassay(Abbot Axsym system) for determination of β2-microglobuline in urine. We examined samples (serum and urine) from 70 participants (35 Psa untreated, 35 healthy control group). RF and CRP are determined with Latex agglutination test in the same participants. Results: From 35 examined patients with Psa, 12 pts showed presence of AAP enzymuria (test sensitivity was 34.28%), 8 pts showed presence of γ-GT (test sensitivity was 22.85%), while the presence of β2-microglobuline in urine was low (test sensitivity 0%). Conclusion: AAP has better sensitivity than γ-GT and β2-microglobuline in the detection of asymptomatic renal endothelial changes in untreated Psa. Correspondence to: Dejan Spasovski, Clinic of Rheumatology, University Clinical Centre-SkopjeVodnjanska 17, 1000 Skopje, Republic of Macedonia, Tel: +389 2 3147-668; E-mail: [email protected]